Zaxis
Tomorrow's Electrophoresis Technology
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Core Products
axis Inc. develops, manufacturers and markets high performance products used to detect and separate various proteins and nucleic acids. These products are used in a protein analysis procedure called electrophoresis, the most commonly used procedure for the analysis of proteins such as cholesterol and nucleic acids including DNA/RNA. Our flagship products include a wide variety of pre-cast protein gels in several gel formulations, percentages and formats. They include Tris-Glycine, Tris-Tricine, Tris-Borate-EDTA (TBE), Tris-Borate-EDTA (TBE) Urea, 2-D and DNA Sequencing. For more information and competitive analyses, visit our product highlights page.
Zaxis will custom manufacture any format glass electrophoresis cassette (including 2D and DNA sequencing) to meet your specifications. Contact us for a quotation.
Benefits of Pre-Cast Gels
Historically, laboratories have easily and inexpensively poured gels for protein samples in a narrow range of molecular sizes. These "Homebrew" or "pour your own" gels do not offer the separation and resolution necessary for the more complicated mixtures of proteins being analyzed today. Additionally, these self-produced gels often have a high "reject rate" and inconsistencies from one pour to another. Zaxis' unique ability to customize and manufacture specific gradient gels provides customers with the benefits of "Homebrew" without the wasted time and money of inconsistant pours.
Zaxis and the Competition
Why are Zaxis' gels better than the competition? We use glass gel cassettes, as opposed to an industry standard of plastic. These glass cassettes offer the advantages of better heat dissipation, reduced leaking of electrical current, and complete recyclability. For more information, be sure to view some of our publications and product highlights.
Electrophoresis Systems
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- System 2000 EP Module
System 2000 EP Module runs one or two gels and fits most major brand cassettes, both 8 x 10 cm and 10 x 10 cm cassettes. It employs a dual wiper seal and utilizes a self clamping guide rail system. The top tank cannot leak dry. System 2000 EP Module provides high visibility for easy loading of sample.
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- System 2000 IEF Upper Buffer Tank
System 2000 IEF Upper Buffer Tank is designed to run twelve microcapillary tube gels for Isoelectric Focusing. System 2000 IEF Upper Buffer Tank is a separate upper chamber which adapts to the System 2000 EP Module. System 2000 IEF Upper Buffer Chamber optimizes results when used in conjunction with the System 2000 EP Module and System 2000 Power Supply.
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- System 2000 Power Supply
System 2000 Power Supply is a variable and programmable low voltage electrophoresis power supply capable of producing up to 350 volts, 250 milliamps and 87.5 watts. It is suited to automatically crossover from one mode of operation to the other when the preset limit has been reached. Effective time management has been added to the design by introducing a dual function autoranging 24 hour up/down timer. System 2000 Power Supply is supplied with four sets of output connectors capable of powering a maximum of four System 2000 EP Modules. System 2000 Power Supply optimizes results when used in conjunction with the System 2000 EP Module.
Zaxis Pre-Cast Gels
Zaxis manufactures a wide range of protein gels available in both 8cm high, 10cm wide and 10cm high, 10cm wide cassettes--depending upon the gel type. They are compatible with all existing electrophoresis tanks. To display detailed information, choose from the following list of gel formulations.
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- Tris-Glycine Pre-Cast Gels
- Tris-Tricine Pre-Cast Gels
- Tris-Borate-EDTA (TBE) Pre-Cast Gels
- Tris-Borate-EDTA (TBE) Urea Pre-Cast Gels
- 2-D Tris-Glycine Pre-Cast Gels
- Mini Z-Quence DNA Sequencing/RNA Analysis Gels
- Z-Quence DNA Sequencing Pre-Cast Gels
- Lipoprotein Fractionation Systems
Tris-Glycine Gels
Zaxis Tris-Glycine gels permit Ultra High Resolution analysis of the full protein and polypeptide molecular weight ranges found in nature. Zaxis provides a full range of polyacrylamide gels: five gradient formats and three non-gradient formats. Results are optimized when used in conjunction with the matched buffers and stains: Zaxis SDS Running Buffer, either Zaxis Tris-HCl-SDS or Zaxis Tris-HCl-SDS-2-mercaptoethnol Sample Preparation Buffer and Zaxis Colloidal Coomasie G-250 Stain, in addition to, the System 2000 EP Module and System 2000 Power Supply. Gels marked with an asterisk (*) only have a 2 month shelf life, are poured to order, and require a minimum order quantity of 20 gels (nominal tower pour).
- Separation Ranges: 5-200 kD
- Formats: 8 x 10 cm, 10 x 10 cm
- Shelf Life: 4 months (* = 2 months)
- Gradient Formats: 4-12%, 4-27%, 4-20%, 10-20% and 17-27%
- Non-Gradient Formats: 6%, 10%, and 16%With 12 Lane Preformed Comb
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8 x 10 cm Tris-Glycine Gels with 4% Stacking Layer
8 x 10 cm glass cassette, 1mm thick, 12 pre-formed wells, 4 month shelf life
| Description | Qty | Price |
| 4-12% Tris-Glycine Gel | 5 pack | $50.00 |
| 4-12% Tris-Glycine Gel | 10 pack | $90.00 |
| 4-20% Tris-Glycine Gel | 5 pack | $50.00 |
| 4-20% Tris-Glycine Gel | 10 pack | $90.00 |
| 8% Tris-Glycine Gel | 10 pack | $90.00 |
| 10% Tris-Glycine Gel | 5 pack | $50.00 |
| 10% Tris-Glycine Gel | 10 pack | $90.00 |
| 12% Tris-Glycine Gel | 5 pack | $50.00 |
| 12% Tris-Glycine Gel | 10 pack | $90.00 |
| 16% Tris-Glycine Gel* | 20 pack | $180.00 |
| 17-27% Tris-Glycine Gel* | 20 pack | $280.00 |
| 10-20% Tris-Glycine Gel | 5 pack | $50.00 |
| 10-20% Tris-Glycine Gel | 10 pack | $90.00 |
10 x 10 cm Tris-Glycine Gels with 4% Stacking Layer
10 x 10 cm glass cassette, 1mm thick, 12 pre-formed wells, 4 month shelf lifeyui
| Description | Qty | Price |
| 4-12% Tris-Glycine Gel | 5 pack | $55.00 |
| 4-12% Tris-Glycine Gel | 10 pack | $95.00 |
| 4-20% Tris-Glycine Gel | 5 pack | $55.00 |
| 4-20% Tris-Glycine Gel | 10 pack | $95.00 |
| 8% Tris-Glycine Gel | 10 pack | $95.00 |
| 10% Tris-Glycine Gel | 5 pack | $55.00 |
| 10% Tris-Glycine Gel | 10 pack | $95.00 |
| 12% Tris-Glycine Gel | 5 pack | $55.00 |
| 12% Tris-Glycine Gel | 10 pack | $95.00 |
| 16% Tris-Glycine Gel | 20 pack | $190.00 |
| 17-27% Tris-Glycine Gel | 20 pack | $290.00 |
| 10-20% Tris-Glycine Gel | 5 pack | $55.00 |
| 10-20% Tris-Glycine Gel | 10 pack | $95.00 |
Running Buffers for Tris-Glycine Gels
For best results, we recommend the following buffers from the Gentaur
| Description | Qty | Price |
| 1X Concentrate Tris-Glycine SDS | 10 L | $42.50 |
| 10X Concentrate Tris-Glycine SDS | 500 ml | $14.00 |
| 10X Concentrate Tris-Glycine Native | 500 ml | $17.50 |
Sample Preparation Buffers for Tris-Glycine Gels
For best results, we recommend the following buffers from the Zaxis Catalog
| Description | Qty | Price |
| 2X Concentrate Tris-HCL-SDS | 25 ml | $10.00 |
| 2X Concentrate Tris-HCI-SDS-2-mercaptoethanol | 25 ml | $10.00 |
| 2X Concentrate Tris-Glycine Native | 25 ml | $10.00 |
Zaxis spacer adapters allow thin 1 mm Zaxis gels to be used with both Novex and Biorad tanks. Zaxis gels should work without adapters on most other vertical mini electrophoresis tanks. For more information, consult our compatibility table. These adapters would gladly be provided at no cost with an initial order of 15 gels or more.
Gel Plate Adapters
For Using Gentaur Gels in Non-Gentaur Modules
| Description | Qty | Price |
| Spacer Adapter - Gentaur Vertical Tank | 1 | $10.00 |
| Spacer Adapter - Gentaur Vertical Tank | 1 | $10.00 |
InstantBlue™ is a ready to use Coomassie protein stain for polyacrylamide gels. Its unique mechanism of action, stains proteins in 15 minutes while leaving a clear background eliminating the need to fix, wash or destain.
Ultra-fast staining and single step procedure
High sensitivity with 5 ng bands detectable
Clear background with high signal to noise ratio
Formulated for safe use and easy disposal
Available in convenient pre-mixed 1 litre volume
Tris-Borate-EDTA Gels
Zaxis Tris-Borate-EDTA (TBE) gels permit Ultra High Resolution analysis of DNA/RNA and native proteins. Zaxis provides a full range of polyacrylamide TBE gels: three gradient formats and one non-gradient formats. Results are optimized with TBE gels when used in conjunction with the native (TBE) buffers and stain.
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- Separation Ranges: 5-200 kD, 10-1,400 base pairs
- Formats: 8 x 10 cm, 10 x 10 cm
- Shelf Life: 6 months
- Gradient Formats: 4-12%, 4-20%
- Non-Gradient Formats: 10%
- 1 small tacking lane, 1 large sample lane for 2D strip
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8 x 10 cm Tris-Borate-EDTA (TBE) Gels with 4% Stacking Layer
8 x 10 cm glass cassette, 1mm thick, 1 small tacking lane, 1 large sample lane for 2D strip, 6 month shelf life
| Description | Qty | Price |
| 4-12% Tris-Borate-EDTA (TBE) Gel | 10 pack | $90.00 |
| 4-20% Tris-Borate-EDTA (TBE) Gel | 10 pack | $90.00 |
| 10% Tris-Borate-EDTA (TBE) Gel | 10 pack | $90.00 |
10 x 10 cm Tris-Borate-EDTA (TBE) Gels with 4% Stacking Layer
10 x 10 cm glass cassette, 1mm thick, 1 small tacking lane, 1 large sample lane for 2D strip, 6 month shelf life
| Description | Qty | Price |
| 4-12% Tris-Borate-EDTA (TBE) Gel | 10 pack | $95.00 |
| 4-20% Tris-Borate-EDTA (TBE) Gel | 10 pack | $95.00 |
| 10% Tris-Borate-EDTA (TBE) Gel | 10 pack | $95.00 |
Running Buffers for Tris-Borate-EDTA Gels
For best results, we recommend the following buffers from the Gentaur Catalog
| Description | Qty | Price |
| 1X Concentrate Tris-Borate-EDTA (TBE) | 10 L | $45.00 |
| 5X Concentrate Tris-Borate-EDTA (TBE) | 1000 ml | $17.50 |
Sample Preparation Buffers for Tris-Borate-EDTA Gels
For best results, we recommend the following buffers from the Gentaur Catalog
| Description | Qty | Price |
| 2X Tris-Borate-EDTA (TBE) | 25 ml | $10.00 |
Tris-Borate-EDTA Urea Gels
These pre-cast polyacrylamide gels are designed for denaturing nucleic acid analyses and perform best in the range of 20-800 bases. They can separate single stranded DNA oligos or RNA into clearly separate bands based on our proprietary chemistry (with 7M Urea) and pouring methods used in their preparation. They are available in 8 x 10 cm and 10 x 10 cm cassettes with 12 preformed wells. When stored at 4° C, these gels have a minimum shelf life of 6 months. For best results, it is recommended that these gels be used with our matching TBE Running Buffer.
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- Separation Ranges: 20-800 base pairs
- Formats: 8 x 10 cm, 10 x 10 cm
- Shelf Life: 6 months
- Gradient Formats: none
- Non-Gradient Formats: 6%, 10%
- With 12 Lane Preformed Comb
8 x 10 cm Tris-Borate-EDTA Urea Gels
8 x 10 cm glass cassette, 1 mm thick, 12 pre-formed wells, 6 month shelf life, for nucleic acid analysis
| Description | Qty | Price |
| 6% Tris-Borate-EDTA (TBE) Urea Gel | 10 pack | $120.00 |
| 10% Tris-Borate-EDTA (TBE) Urea Gel | 10 pack | $120.00 |
10 x 10 cm glass cassette, 1 mm thick, 12 pre-formed wells, 6 month shelf life, for nucleic acid analysis
| Description | Qty | Price |
| 6% Tris-Borate-EDTA (TBE) Urea Gel | 10 pack | $120.00 |
| 10% Tris-Borate-EDTA (TBE) Urea Gel | 10 pack | $120.00 |
For best results, we recommend the following buffers from the Gentaur Catalog
| Description | Qty | Price |
| DNA Matched Long Life Running Buffer | 10 L | $65.00 |
2-D Gels
Zaxis 2D gels are pre-cast second dimension Tris-Glycine gradient mini-gels with a 1 cm 4% stacking layer, and are to be used in the second phase of a two stage electrophoresis process. They are manufactured as pre-poured glass cassettes with 1 mm gel thickness, a single molecular weight reference sample lane and an overall cassette dimension of 10 x 10 cm to fit most vertical electrophoresis systems.
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- Separation Ranges: 6-240 kD
- Formats: 10 x 10 cm
- Shelf Life: 4 months
- Gradient Formats: 8-16%, 10-20%
- Non-Gradient Formats: none
- With 12 Lane Preformed Comb
10 x 10 cm 2-D Tris-Glycine Gels with 4% Stacking Layer
10 x 10 cm glass cassette, 1mm thick, single sample reference lane, 4 month shelf life
| Description | Qty | Price |
| 8-16% 2-D Tris-Glycine Gel | 10 pack | $120.00 |
| 10-20% 2-D Tris-Glycine Gel | 10 pack | $120.00 |
For best results, we recommend the following buffers from the Gentaur Catalog
| Description | Qty | Price |
| 1X Concentrate Tris-Glycine SDS | 10 L | $42.50 |
| 10X Concentrate Tris-Glycine SDS | 500 ml | $14.00 |
| 10X Concentrate Tris-Glycine Native | 500 ml | $17.50 |
For best results, we recommend the following buffers from the Gentaur Catalog
| Description | Qty | Price |
| 2X Concentrate Tris-HCL-SDS | 25 ml | $10.00 |
| 2X Concentrate Tris-HCI-SDS-2-mercaptoethanol | 25 ml | $10.00 |
| 2X Concentrate Tris-Glycine Native | 25 ml | $10.00 |
Mini Z-Quence DNA Sequencing Gels
Mini Z-Quence DNA Sequencing/RNA Analysis Gels are available in a 10x12.5cm all-glass cassette that fits Novex QuickPoint Tanks. The plates are constructed of 1.5 mm glass with a 0.25 mm poured gel thickness. Using proprietary chemistry and pouring methods, the shelf life of the pre-cast gels, when stored at 4° C, are guaranteed for 6 months.qd
- Format: 10 x 12.5 cm
- Shelf Life: 6 months
- Non-Gradient Formats: 6%, 8%, 10%
- (custom percentages available upon request)
Mini Z-Quence DNA Sequencing/RNA Analysis Gels
16 preformed wells (1.9µL/well capacity), 0.25mm gel, 6 mo. shelf life
| Description | Qty | Price |
| 6% Mini Z-Quence Gels | 5 pack | $125.00 |
| 8% Mini Z-Quence Gels | 5 pack | $125.00 |
| 10% Mini Z-Quence Gels | 5 pack | $125.00 |
For best results, we recommend the following buffer from the Zaxis Catalog
| Description | Qty | Price |
| TBE Matched Long Life Running Buffer | 4X 2.5L | $65.00 |
Z-Quence DNA Sequencing Gels
Pre-cast Long Life DNA Sequencing Gels are available in two standard plate sizes and as a self pouring solution. The plates are constructed of 1.5 mm glass with a 0.4 mm poured gel thickness, and come without a comb (flat-top). Using proprietary chemistry and pouring methods, the shelf life of the pre-cast gels, when stored at 4° C, are guaranteed for 6 months.
The thinner pre-cast plate design allows for better cooling and faster run times than the more traditional self-poured 6 mm glass plates. The resulting plate is more flexible, allowing the user to more finely tune tension across the length of a sharks tooth comb which results in less lane-to-lane leakage than the heavier plates. Note that special plate tension adapters and gaskets are required to use these thinner plates with the Hoefer and LTI vertical electrophoresis systems. These adapters and gaskets would be at no charge with an initial customer order of a minimum of 15 pre-cast gels.
- Separation Ranges: 20-800 base pairs
- Formats: 333 x 419 mm ( Hoefer SQ3, LTI S2), 197 x 363 mm (LTI SA-32)
- Shelf Life: 6 months
- Gradient Formats: none
- Non-Gradient Formats: 6%
Z-Quence Long Life Pre-Cast DNA Sequencing Gels
1.5mm glass, 0.4mm gel, flat top gel, combs extra, 6 month shelf life
| Description | Qty | Price |
| 6%, S2/SQ3, 333 x 419 mm | 5 pack | $175.00 |
| 6%, SA32, 197 x 363 mm | 5 pack | $175.00 |
Z-Quence Long Life DNA Sequencing Gel Solution
6 month shelf life, for self-pouring
| Description | Qty | Price |
| 6% Gel Solution | 100 ml | $98.80 |
Running Buffers for DNA Sequencing Gels
For best results, we recommend the following buffers from the Zaxis Catalog
| Description | Qty | Price |
| DNA Matched Long Life Running Buffer | 10 L | $65.00 |
Adapters
Zaxis pre-cast DNA electrophoresis gels are cast between two 1.5 mm glass sheets, allowing for better cooling, faster run times, and less lane-to-lane leakage. As a result of using glass so thin in comparison to the standard 6 mm, it is an absolute requirement that you use a flat surface gasket. The Z-Quence Universal Adjustment Kit includes the necessary gasket and a tension adjustment device that compensates for variance in comb thickness. When used with Zaxis gels, this kit nearly eliminates lane leakage.
Gel Plate Adapters
For Using Zaxis Gels in Non-Zaxis Modules
| Description | Qty | Price |
| Tension Adapter Kit for LTI-S2 | 1 | $125.00 |
| Tension Adapter Kit for Hoefer SQ3 | 1 | $125.00 |
| Tension Adapter Kit for LTI-SA32 | 1 | $125.00 |
Lipoprotein Fractionation Supplies
Lipoprotein Fractionation Kit
| Description | Qty | Price |
| Lipoprotein Ten Gel Complete Kit Includes ten gels for a total of 100 serum samples for fractionation and analysis of lipoproteins, sample SuperBlue additive, running buffers, LFS post stain & destain solutions. | 1 kit | $295.00 |
| Additional Lipoprotein Gels Ten serum samples - per gel only | 1 gel | $25.00 |
Lipoprotein Fractionation Buffers and Stains
| Description | Qty | Price |
| Colloidal Lipoprotein Post Stain | 500 mL | $65.00 |
| Lipoprotein Destain | 1000 mL | $33.00 |
| 1X TBE Top Tank Running Buffer (pH 8.7) | 1000 mL | $15.50 |
| 1X TBE Bottom Tank Running Buffer (pH 8.3) | 1000 mL | $15.50 |
| 1X Sample Prep SuperBlue Additive | 25 mL | $19.50 |
Molecular Biology Grade Water
Zaxis DNase/RNase Free Water is excellent for use in PCR and sequence reactions where RNA or DNA cleavace is strictly prohibited. 18 m-ohm deionized, ozonated, UV pasteurized water treated with 0.001% diethylpyrocarbonate (DEPC). Filtered through 5,000 MW cut off filter and autoclaved to yield a sterile solution completely free of detectable DNase (both exo and endo) and RNase.
Zaxis PCR Water is ideal for use in polymerase chain reactions (PCR). 18 m-ohm deionized, ozonated, UV pasteurized water filtered through a 5,000 MW cut off filter.
Dnase / Rnase Free Water, PCR Water
| Description | Qty | Price |
| Water, DNase/RNase free | 500 ml | $25.00 |
| Water, DNase/RNase free | 1 L | $37.50 |
| Water, for PCR assay | 1.5 ml | $10.50 |
Empty Glass Cassettes and Plastic Combs
These empty cassettes and accompanying combs are the same we use for our gels. They are provided here in the event that a customer would prefer to pour his or her own gels.
Empty Glass Cassettes
| Description | Qty | Price |
| 8x10 cm Glass Cassette | 10 pack | $59.50 |
| 10x10 cm Glass Cassette | 10 pack | $69.50 |
| Description | Qty | Price |
| 12 Well Comb | 10 combs | $45.00 |
| 10 Well Comb | 10 combs | $45.00 |
